• Histological section of rabbit retina, showing its different layers Hematoxylin-eosin staining. Photo: P. Fernández-Martínez
  • 12 DIV rat hippocampal culture. Green, MAP2B . Blue; nuclear stainning with DAPI. Size 6mm^2. Photo: G. Cuesto & P. Fernández-Martínez
  • Red DiI and green DiO stainning of three hippocampal CA1 rat (6 months) neurons using the Gene Gun technique. Blue: Nuclear stainning by DAPI. stratum pyramidale. Photo: N. Domínguez-Iturza & G. Mata & L. Enríquez
  • Red DiI stainning of a hippocampal CA1 rat (6 months) neuron using the Gene Gun technique. Blue: Nuclear stainning by DAPI. stratum pyramidale. Photo: N. Domínguez-Iturza & L. Enríquez & G. Mata
  • DiI staining of the mouse hippocampus using the Gene gun. Pyramidal CA1 hippocampal neuron. Photo: N. Domínguez-Iturza & L. Enríquez & G. Mata
  • DiI stanning of a TG2576 transgenic mouse that overexpress a mutant form of human APP protein. CA1 hippocampal neurons stainned using a Gene Gun. Photo: N. Domínguez-Iturza & L. Enríquez & G. Mata
  • DiI staining of the mouse hippocampal slice using the Gene gun. Mosaic picture of 1500x1500 um individual pictures employong the Matrix (LasAF ; Leica) controlling a SP5 confocal microscope. Photo: N. Domínguez-Iturza & L. Enríquez & G. Mata
  • Golgi staining of the rat hippocampus. Pyramidal neurons in CA1. Photo: L. Enríquez-Barreto.
  • Hippocampal neuron in culture transfected with an Actin-GFP vector. Photo: L. Enríquez-Barreto & G. Cuesto.
  • Basal dendritic segment of a pyramidal hippocampal neuron in CA1. Diolistic Labeling. Photo: N. Domínguez-Iturza & L. Enríquez-Barreto.
  • 12 DIV rat hippocampal culture. Red, MAP2B . Green; nuclear stainning with DAPI. Size 6mm^2. Photo: G. Cuesto & P. Fernández-Martínez
  • Hippocampal neuron in culture transfected with an Actin-GFP vector. Photo: L. Enríquez-Barreto & G. Cuesto.
  • Hippocampal neuron in culture transfected with an Actin-GFP vector. Photo: L. Enríquez-Barreto & G. Cuesto.

These images and movies have been acquired with a Leica SP5 Confocal (Leica Microsystems Heidelberg, GmbH).


We like to thank to Paco Porto and all the technical service of Leica Spain for their help and kindness.

Movies

Movie by L. Enríquez-Barreto
Movie by L. Enríquez-Barreto

Motility and dynamics of hipocampal neuron spines in cultures. Neurons grew for 18 days. Cells were treated with the PTD4-PI3KAc peptide. Total length 30 minutes in 2 minutes time samples.